News News

Optimization of protocols for DNA extraction from fecal samples

High-throughput sequencing offers new possibilities in molecular ecology and conservation studies. However, its potential has not yet become fully exploited for noninvasive studies of free–ranging animals, such as those based on feces. High–throughput sequencing allows sequencing of short DNA fragments and could allow simultaneous genotyping of a very large number of samples and markers at a low cost. The application of high throughput genotyping to fecal samples from wildlife has been hindered by several labor intensive steps. Alternative protocols which could allow higher throughput were evaluated for two of these steps: sample collection and DNA extraction. Two different field sampling and seven different DNA extraction methods were tested on grey wolf (Canis lupus) feces. There was high variation in genotyping success rates. The field sampling method based on surface swabbing performed much worse than the extraction from a fecal fragment. In addition, there is a lot of room for improvement in the DNA extraction step. Optimization of protocols can lead to very much more efficient, cheaper and higher throughput noninvasive monitoring. Selection of appropriate markers is still of paramount importance to increase genotyping success. informacion[at]ebd.csic.es: Sarabia et al (2020) Towards high-throughput analyses of fecal samples from wildlife. Animal Biodiver Conserv 43.2: 271–283 Doi 10.32800/abc.2020.43.0271


http://abc.museucienciesjournals.cat/volum-43-2-2020/towards-high-throughput-analyses-of-fecal-samples-from-wildlife/?lang=en
Average (0 Votes)

Latest News Latest News

Back

The life cycle of an alien boatman

The life cycle of an alien boatman

Trichocorixa verticalis (Corixidae) is native to North America but is well established as an alien in the Western Mediterranean region, where it is invasive in permanent coastal wetlands with high salinities. The annual cycle and generation time of T. verticalis was investigated in the introduced range in south-west Spain, through a combination of field surveys and laboratory experiments. Field surveys were conducted on a monthly basis over one year in three saline fish ponds in Doñana and four hypersaline salt ponds in the Odiel marshes. Adults were present all year round, whereas nymphs were only absent in August, when temperatures and salinities were high. Adult sex ratios were idiosyncratic and often male- or female- biased for a given location and month. Adults were smaller during summer months. Laboratory experiments revealed an oviposition rate of 11.5 eggs per day and a generation time of about 54 days from egg to adult, suggesting T. verticalis may complete around six generations per year in permanent wetlands. A combination of a high oviposition rate and continuous reproduction throughout the year give T. verticalis an advantage over native corixid competitors (Sigara spp) and appear to explain the success of this alien aquatic insect. informacion[at]ebd.csic.es: Céspedes et al (2018) The life cycle of the alien boatman Trichocorixa verticalis (Hemiptera, Corixidae) in saline and hypersaline wetlands of south-west Spain. Hydrobiol DOI 0.1007/s10750-018-3782-x


https://link.springer.com/article/10.1007/s10750-018-3782-x