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Optimization of protocols for DNA extraction from fecal samples

High-throughput sequencing offers new possibilities in molecular ecology and conservation studies. However, its potential has not yet become fully exploited for noninvasive studies of free–ranging animals, such as those based on feces. High–throughput sequencing allows sequencing of short DNA fragments and could allow simultaneous genotyping of a very large number of samples and markers at a low cost. The application of high throughput genotyping to fecal samples from wildlife has been hindered by several labor intensive steps. Alternative protocols which could allow higher throughput were evaluated for two of these steps: sample collection and DNA extraction. Two different field sampling and seven different DNA extraction methods were tested on grey wolf (Canis lupus) feces. There was high variation in genotyping success rates. The field sampling method based on surface swabbing performed much worse than the extraction from a fecal fragment. In addition, there is a lot of room for improvement in the DNA extraction step. Optimization of protocols can lead to very much more efficient, cheaper and higher throughput noninvasive monitoring. Selection of appropriate markers is still of paramount importance to increase genotyping success. informacion[at]ebd.csic.es: Sarabia et al (2020) Towards high-throughput analyses of fecal samples from wildlife. Animal Biodiver Conserv 43.2: 271–283 Doi 10.32800/abc.2020.43.0271


http://abc.museucienciesjournals.cat/volum-43-2-2020/towards-high-throughput-analyses-of-fecal-samples-from-wildlife/?lang=en
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The biochemical basis of the sexual ornaments of the red grouse unravels their informative content

The biochemical basis of the sexual ornaments of the red grouse unravels their informative content

The carotenoid-based ornaments displayed by many birds often play key roles in social and sexual signalling, revealing information about individual quality. Understanding the mechanisms of coloured trait production and maintenance requires an accurate description of their composition and of the physiological pathways involved in their expression. Carotenoid-based colouration has been extensively studied in birds, but such information is often lacking for coloured integuments other than feathers, such as fleshy carotenoid based ornaments. Authors report the carotenoid composition of the combs of the red grouse, a sexual trait that honestly reveals individual quality. It was found that comb pigmentation was primarily the result of two red ketocarotenoids (astaxanthin and papilioerythrinone), which are synthesised from their dietary precursors (zeaxanthin and lutein) directly at the comb integument. These red ketocarotenoids are largely deposited esterified with fatty acids. Astaxanthin concentration in the comb was found to negatively correlate with retinol levels but positively correlate with tocopherol levels. Considering evidence from this and other studied species, authors suggest that carotenoid esterification is a characteristic of coloured fleshy integuments, probably affecting pigment stability and colouration in living tissues, with subsequent effects on their signalling role and maintenance costs. Honest signalling via comb colouration appears mostly related to potential allocation trade-offs of some specific dietary precursors or to the capacity of individuals to manage the redox reactions interfering with carotenoid metabolism. informacion[at]ebd.csic.es: Pérez-Rodríguez et al (2016) Carotenoid profile and vitamins in the combs of the red grouse (Lagopus lagopus scoticus): implications for the honesty of a sexual signal. J Ornithol 157:145–153 DOI 10.1007/s10336-015-1261-y


http://link.springer.com/article/10.1007/s10336-015-1261-y