News News

Optimization of protocols for DNA extraction from fecal samples

High-throughput sequencing offers new possibilities in molecular ecology and conservation studies. However, its potential has not yet become fully exploited for noninvasive studies of free–ranging animals, such as those based on feces. High–throughput sequencing allows sequencing of short DNA fragments and could allow simultaneous genotyping of a very large number of samples and markers at a low cost. The application of high throughput genotyping to fecal samples from wildlife has been hindered by several labor intensive steps. Alternative protocols which could allow higher throughput were evaluated for two of these steps: sample collection and DNA extraction. Two different field sampling and seven different DNA extraction methods were tested on grey wolf (Canis lupus) feces. There was high variation in genotyping success rates. The field sampling method based on surface swabbing performed much worse than the extraction from a fecal fragment. In addition, there is a lot of room for improvement in the DNA extraction step. Optimization of protocols can lead to very much more efficient, cheaper and higher throughput noninvasive monitoring. Selection of appropriate markers is still of paramount importance to increase genotyping success. informacion[at]ebd.csic.es: Sarabia et al (2020) Towards high-throughput analyses of fecal samples from wildlife. Animal Biodiver Conserv 43.2: 271–283 Doi 10.32800/abc.2020.43.0271


http://abc.museucienciesjournals.cat/volum-43-2-2020/towards-high-throughput-analyses-of-fecal-samples-from-wildlife/?lang=en
Average (0 Votes)

Latest News Latest News

When remote sensing and field approaches combine

Implementing long-term monitoring programs that effectively inform conservation plans is a top priority in environmental management. In floodplain forests, historical pressures interplay with the...

Pigmentation plasticity in newt larvae

In heterogeneous environments, the capacity for colour change can be a valuable adaptation enhancing crypsis against predators. Alternatively, organisms might achieve concealment by evolving...

Shape matters: colour patterns as signals of quality

Colour patterns, e.g. irregular, spotted or barred forms, are widespread in the animal kingdom, yet their potential role as signals of quality has been mostly neglected. However, a review of the...

Light pollution and seabird fledglings

One of the most critical phases in the life of petrels is at fledging when young birds pass from parental dependence on land to an independent life at sea. To mitigate mortality at this time,...