News News

Optimization of protocols for DNA extraction from fecal samples

High-throughput sequencing offers new possibilities in molecular ecology and conservation studies. However, its potential has not yet become fully exploited for noninvasive studies of free–ranging animals, such as those based on feces. High–throughput sequencing allows sequencing of short DNA fragments and could allow simultaneous genotyping of a very large number of samples and markers at a low cost. The application of high throughput genotyping to fecal samples from wildlife has been hindered by several labor intensive steps. Alternative protocols which could allow higher throughput were evaluated for two of these steps: sample collection and DNA extraction. Two different field sampling and seven different DNA extraction methods were tested on grey wolf (Canis lupus) feces. There was high variation in genotyping success rates. The field sampling method based on surface swabbing performed much worse than the extraction from a fecal fragment. In addition, there is a lot of room for improvement in the DNA extraction step. Optimization of protocols can lead to very much more efficient, cheaper and higher throughput noninvasive monitoring. Selection of appropriate markers is still of paramount importance to increase genotyping success. informacion[at]ebd.csic.es: Sarabia et al (2020) Towards high-throughput analyses of fecal samples from wildlife. Animal Biodiver Conserv 43.2: 271–283 Doi 10.32800/abc.2020.43.0271


http://abc.museucienciesjournals.cat/volum-43-2-2020/towards-high-throughput-analyses-of-fecal-samples-from-wildlife/?lang=en
Average (0 Votes)

Latest News Latest News

Demographic processes in colonial vulture species

Understanding how density dependence modifies demographic parameters in long-lived vertebrates is a challenge for ecologists. A number of studies have highlighted the importance of habitat...

Analysis of the effects of an extreme climatic event on scrub communities

The effects of an extreme climatic event on shrub species were assessed by analyzing their leaves, roots, seeds and stem.The long-term monitoring of scrub communities allowed to evidence the...

Identificación de regiones genómicas bajo selección en perros domésticos

Identification of the genomic regions under selection during dog domestication is extremely challenging because the demographic fluctuations associated with domestication can produce signals in...

Oceanographic drivers and mistiming processes shape breeding success in a seabird

Understanding the processes driving seabirds' reproductive performance through trophic interactions requires the identification of seasonal pulses in marine productivity. In this study, it was...

Parasites help brine shrimp cope with arsenic habitat contamination

Do parasites weaken their hosts’ resilience to environmental stress? Not always, according to this study. Rather than weakening its brine shrimp intermediate host, tapeworm infection enhances the...