The aquatic ecosystems of Doñana and those of its watersheds are highly threatened by multiple human pressures. Among them, the strong development of agriculture and the increase of population have been the main causes of the loss of quantity and quality of freshwaters during the last decades. Nitrate pollution is one of the main problems affecting both surface and groundwaters in Doñana and its surroundings. Previous studies have shown that the Doñana aquifer receives nitrates due to the...