Characteristics

Stable isotopes are non-radioactive atoms of a particular chemical element which have the same number of protons but different number of neutrons. The relative abundance of the heavy compared with the light isotope (e.g. 13C/12C) is given the name isotopic signature or fingerprint and is represented by a delta symbol (δ) by means of the ratio of this proportion in the sample, with respect to international standards, giving the results in units per thousand (‰).

 

The Doñana Biological Station (EBD) Stable Isotope Laboratory (LIE) is equipped with a Mass Spectrometry system, supplied by Thermo Fisher Scientific, to measure the isotope ratios 13C/12C, 15N/14N, 18O/16O, 34S/32S and 2H/1H in organic and inorganic, solid or liquid samples.

 

This Laboratory was founded thanks to funding from the project: “Adapting to Global Change in the Mediterranean hotspot: from genes to ecosystems (EcoGenes)”.

 

ecogenes

 

Here we give you a virtual tour of the EBD, funded through EcoGenes, in which you can see all the laboratories including the LIE.

 

visita

 

 

 


Equipment

DELTA V Advantage IRMS in continuous flow mode, with a system of 5 collectors for measuring isotopic ratios in N2, CO, NO, O2, CO2, N2O, SO2 and H2.

 

IRMS

 

Flash HT Plus Elemental Analyzer connected to an IRMS via the Conflo IV. With two furnaces installed for two modes of operation: “Dynamic Flash Combustion” for isotopic analysis of C, N and S; and “High Temperature Conversion” for isotopic analysis of O and H.

 

EA

 

Picarro L2130-i CRDS (Cavity Ring Down Spectroscopy) Laser Spectrometer for continuously measuring the stable isotopes of Hydrogen and Oxygen in water and water vapour.

 

Laser_Picarro

 

 

LEER

Latest News

New Paper!: Diet of bottlenose dolphins (Tursiops truncatus) from the Gulf of Cadiz: Insights from stomach content and stable isotope analyses

 

New Paper!: Trophic habits of an abundant shark in the northwestern Mediterranean Sea using an isotopic non-lethal approach

 

New laboratory e-mail: lie@ebd.csic.es, for any request!

 

2017 New analysis fees published on "Services" tab: New Fees

 

New Paper!: Feeding ecology and trophic relationships of pelagic sharks and billfishes coexisting in the central eastern Pacific Ocean

 

New Paper!: Feeding habits of three Batoids in the Levantine Sea (north-eastern Mediterranean Sea) based on stomach content and isotopic data

 

New Paper!: Lipid effects on isotopic values in bottlenose dolphins (Tursiops truncatus) and their prey with implications for diet assessment

 

Breaking news!: Why we need a centralized repository for isotopic data (Read more)

 

Research report 2016 published Report 2016

 

New Paper!: Intra- and interspecific niche partitioning in striped and common dolphins inhabiting the southwestern Mediterranean Sea

 

New scientific project: Understanding trophic ecology of the California kingsnake, a new invasive species in Gran Canaria (Canary Islands, Spain)

 

New Paper!: Feeding ecology of two demersal opportunistic predators coexisting in the northwestern Mediterranean Sea

 

New Paper!: Assessing the trophic ecology of three sympatric squid in the marine ecosystem off the Patagonian Shelf by combining stomach content and stable isotopic analyses

 

An online temperature-controlled vacuum equilibration preparation system for the measurement of δ2H values of nonexchangeable-H and of δ18O values in organic materials by isotope-ratio mass spectrometry (Read more)