• Introduction
  • Instructors
  • Program
  • Registration
  • Accommodation
  • Supplementary information

Course overview and announcement




Application period is now closed for the 4th edition of the STABLE ISOTOPE COURSE IN ECOLOGY AND ENVIRONMENTAL SCIENCES, which will be held on 22nd-25th February 2022, organized by the Laboratorio de Isótopos Estables at Estación Biológica de Doñana  (LIE-EBD, CSIC, Sevilla).

Following the success of the FIRST, SECOND and THIRD EDITIONS, this course will develop a conceptual, quantitative, and thorough understanding of principles of stable isotopes and its applications in aquatic and terrestrial ecological studies. The course includes an introductory session on the applications of stable isotopes to ecological and environmental studies including a general introduction to the stable isotope biochemistry and ecology, trophic and spatial applications and general laboratory methodologies. In the present edition of the course, the rest of the program will be focused on the analyses of stable isotopes on compound specifics (CSIA), and their applications including various thematic lectures: introduction to CSIA, technical equipment, steps prior to stable isotope analyses, applications to different areas with special attention to environmental sciences.

The course also includes some practical sessions in the Laboratory (LIE) and a visit to the EBD Natural History Scientific Collection, where participants could better understand the value of these scientific infrastructures for long term studies using stable isotope analyses. The course will finish with a field trip to Doñana National Park.


The course is open to the participation of graduate and PhD students, professors, researchers and laboratory technicians interested in learning more about the stable isotope approach in general and CSIA in particular to solve ecological problems.  We look forward to receive applications from people with backgrounds in disciplines including animal and plant physiology, ecology and ecosystem science, biogeochemistry, anthropology, atmospheric science, marine science, oceanography, paleontology, forensic science, industry and geology.


Ediciones anteriores: (LINK)


Sponsored by:





Organizing committee

Manuela G. Forero (Chair) Researcher at the EBD-CSIC

Sarai López (Coordinator) Technician at LIE, EBD-CSIC

Susana Carrasco (Coordinator) Technician at LIE, EBD-CSIC


In collaboration with Coordination office EBD-CSIC




Joan Giménez

Postdoctoral Researcher

Institut de Ciències del Mar (ICM-CSIC)

Passeig Marítim de la Barceloneta, 37-49.
08003 Barcelona (Spain)
T. +34 93 230 95 00

email: joan.gimenez@csic.es


José A. González Pérez

Scientific Researcher

Laboratorio de Isótopos Estables del IRNAS (LIE-IRNAS)

Departamento de. Biogeoquímica, Ecología Vegetal y Microbiana
Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS-CSIC)

Av. Reina Mercedes, 10. 41012 Sevilla (España)

T. 954624711

email: jag@irnase.csic.es


Manuela G. Forero

Scientific Researcher and Scientific Manager at LIE

Departamento de Biología de la Conservación.

Responsable Científico del Laboratorio de Isótopos Estables (LIE-EBD).

Estación Biológica de Doñana (EBD-CSIC)

Avenida de Américo Vespucio, 26

41092 Sevilla, Spain

email: gforero@ebd.csic.es


Keith A. Hobson


Department of Biology

Biological and Geological Sciences Building

Western University, 1151 Richmond St., London, Ontario

Canada N6A 5B7

email: khobson6@uwo.ca


Daniel Carrizo

Scientific Researcher
Unidad de Excelencia María de Maeztu
Centro de Astrobiología (INTA-CSIC)
Instituto Nacional de Técnica Aeroespacial Ctra de Torrejón a Ajalvir, km 4
28850 Torrejón de Ardoz, Madrid Spain
Tel: (+34) 692521256

email: dcarrizo@cab.inta-csic.es


Antonio Bode

Research Professor

Instituto Español de Oceanografía (IEO-CSIC)

Centro Oceanográfico de A Coruña

15001 A Coruña, Spain

email: antonio.bode@ieo.es


Martin J. Kainz

Scientific director and working group leader

WasserCluster Lunz - Biologische Station

Dr. Carl Kupelwieser Promenade 5

A-3293 Lunx am See, Austria

email: martin.kainz@donau-uni.ac.at


Ricardo Álvarez Alonso

Sales Representative

Isotope Ratio Mass Spectrometry & High Resolution ICP-MS

Thermo Fisher Scientific

email: ricardo.alvarez@thermofisher.com


Antonio Carreira

Air Liquide SWE Market Manager

Air Liquide Specialty gases International expert


email: antonio.carreira@airliquide.com


Sarai López y Susana Carrasco

Laboratory Technicians at LIE

Laboratorio de Isótopos Estables (LIE-EBD)

Estación Biológica de Doñana, (EBD-CSIC)

Avenida de Américo Vespucio, 26

41092 Sevilla, Spain

e-mail: lie@ebd.csic.es; sarailopez@ebd.csic.es; susana@ebd.csic.es


Carlos Urdiales

Scientific Researcher

Department of Evolutive Ecology & Scientific Collections
Estación Biológica de Doñaña (EBD-CSIC)
41092 Sevilla, Spain
email: colecciones@ebd.csic.es; urdiales@ebd.csic.es


Seth Newsome

Associate Professor, Biology

Associate Director, Center for Stable Isotopes
University of New Mexico
Department of Biology, MSC03-2020
Albuquerque, NM 87131, USA

email: newsome@unm.edu






































The program includes theoretical classes and practical sessions in theLaboratory (LIE-EBD), visit to the EBD Natural History Scientific Collection and a field trip toDoñana National Park (Huelva)


You can download the full program here: pdf



Social events:


- Tuesday 22nd "Tapas in Sevilla downtown" (OPTIONAL, NOT INCLUDED IN THE REGISTRATION COSTS)


- Friday 25th "Field trip to Doñana National Park and lunch in Matalascañas" (LUNCH NOT INCLUDED IN THE REGISTRATION COSTS)





As indicated in the Application form, applicants should provide an expression of interest (max 150 words) and a short background in stable isotopes analyses and their applications in ecological and environmental studies, and related publications (max 200 words).


The number of participants is limited. The Organizing Committee may accept or reject the applications without debate. Once admission has been notified, participants will have 10 business days to make the payment via bank transfer and send proof of payment. Bank details will be provided by the person of contact after admission. The definitive list of assistants will be available starting February 1st.


Fees: 250€


* Accommodation and food regime during the course are not included in the fees.

** Transfer from Seville to Doñana National Park and back is included in the course fees.



This course will be subjected to the COVID-19 laws of Spain and the EU.  These pandemic rules may include or require:

- Official internationally accepted Proof of Vaccination

- A negative PCR test before traveling

- Restriction on travelers coming from outside of Spain or the EU


Registrations cancelled because of COVID-19 regulations will be refunded.


Instead of these potential COVID-19 laws, and to have a COVID-19 free course, we ask you for a COVID-19 vaccination certificate or a recent negative COVID-19 test, which you must provide along with the registration form.



Please, for registration fill the application form: and send to the lab email: lie@ebd.csic.es








The course will be held at the Estación Biológica de Doñana CSIC: C/ Americo Vespucio, 26, 41092, Sevilla; España, and the Reserva Biológica de Doñana: Carretera de Matalascañas s/n 21760. Matalascañas. Huelva (Spain).




Below you will find a short list of accommodations in Seville. No agreements have been taken with hotels; reservation can be done through the hotel’s website or web booking services.



Closer (walking distance):


Hotel Exe Isla Cartuja


Barceló Sevilla Renacimiento












You can find information about how to get to Sevilla by plain and train (here)


For our visit to Reserva Biológica de Doñana  please read carefully our rules of use.



"Tapas" on Tuesday: Contacto y Reservas - Flores Gourmet


Coffee and lunch: pdf












Manuela Forero

Tlno. +34 95 4466700 ext.

e-mail: gforero@ebd.csic.es





Blackwell West, J.B., Bowen, G.J., Dawson, T.E., Tu, K.P. 2010. Isoscapes. Understanding movement, pattern, and process on Earth through isotope mapping. Springer

Clark, I. & Fritz, P. 1997. Environmental Isotopes in Hydrogeology. CRS Press

Dawson, T.E. & Siegwolf, R.T.W. 2007. Stable isotopes as indicators of Ecological Change. Academic Press

Hobson, K.A. & Wassenaar, L.I. 2008. Tracking animal migration with stable isotopes. Academic Press

Fry, B. 2006. Stable Isotope Ecology. Springer

Michener, R. & Lajtha K. 2005. Stable Isotopes in Ecolgoy and Environmental Science.



Journal papers:


Boecklen, W.J., Yarnes, C.T., Cook, B.A., James, A.C. 2011. On the use of stable Isotopes in Trophic Ecology. Annual Review of Ecology, Evolution, and Systematics Vol. 42: 411-440


Parnell, A.C., Phillips, D.L., Bearhop, S., Semmens, B.X., Ward, E.J., Moore, J.W., Jackson, A.L., Grey, J., Kelly, D.J., Inger, R., et al (2013). Bayesian stable isotope mixing models. Environmetrics, 24(6), 387-399.


Parnell, A.C., Inger R., Bearhop, S. & Jackson, A.L. 2010. Source partioning using stable isotopes: coping with too much variation. PLoS ONE, 5(3), e9672 . doi


Semmens, B.X., Ward, E.J., Parnell, A.C., Phillips, D.L., Bearhop, S., Inger, R., Jackson, A., Moore, J.W. (2013). Statistical basis and outputs of stable isotope mixing models: Comment on Fry (2013). Marine Ecology Progress Series, 490, 285-289.



Related web pages:


LIMS for Light Stable Isotopes







Data Analysis Methods and Models for Stable Isotopes



Stable Isotope Network



Commission on Isotopic Abundances and Atomic Weights (CIAAW)



IAEA (Reference products for environment and trade)



Isotopic Reference Materials USGS