Latest News Latest News

Las altas temperaturas están provocando que las lagunas y las marismas de Doñana pierdan agua rápidamente

La superficie inundada en la marisma es de un 78% pero la profundidad es escasa. Por otra parte, sólo el 1,9% de las lagunas temporales están inundadas. Las precipitaciones crean una oportunidad...

Traffic noise causes lifelong harm to baby birds

A study with CSIC participation reveals for the first time that car noise harms individuals throughout their lifetime even years after exposure

Illegal wildlife trade, a serious problem for biodiversity and human health

A research team led by the Doñana BIological Station and the University Pablo de Olavide have detected wild-caught pets in 95% of the localities in the Neotropic and warns of the risk of zoonotic...

Urbanization and loss of woody vegetation are changing key traits of arthropod communities

Urbanization is favouring smaller beetle species and larger spider species with greater dispersal capacity.

The loss of woody areas is linked to a decline in the duration of the activity...

Asset Publisher Asset Publisher

Back

Noninvasive sampling of saliva for genetic analysis in ecology

Noninvasive sampling of saliva for genetic analysis in ecology

Saliva is a secretion rich in epithelial cells and an excellent source of DNA for genetic analysis. Attempts to identify wild vertebrates from noninvasive samples of saliva have been restricted to searching for food remains recently handled by target species. This approach often requires close tracking of animals, which is unfeasible for most species and may explain why saliva is seldom considered in ecological studies. Authors develop a noninvasive method of collection that combines baits and porous materials able to capture saliva. Its potential in optimal conditions has been reported using confined dogs and collecting saliva early after deposition. Mean DNA concentration in saliva extracts was high (14 ng µl-1), whereas species (85%) and individual identification rates (90%) were as high as, or higher than, those reported for other kinds of noninvasive samples such as hair, urine or faeces. Genotyping errors (2%) and mean genotyping effort (2 replicates) remained at very low levels. The procedure could advantageously allow detection of socially low-ranked individuals underrepresented in faecal or urine samples associated with marking behaviour. Once adapted and refined, this technique could yield high rates of individual identification of wild vertebrates in ecological field studies requiring noninvasive sampling. informacion[at]ebd.csic.es: Lobo et al (2015) A new method for noninvasive genetic sampling of saliva in ecological research. PLoS ONE 10 (10) DOI: 10.1371/journal.pone.0139765


http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0139765