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LC-MS determination of catecholamines and related metabolites in red deer urine and hair

A novel analytical methodology for the determination and extraction of catecholamines (dopamine, epinephrine and norepinephrine) and their metabolites DL-3,4-dihydroxyphenyl glycol and DL-3,4-dihydroxymandelic acid by LC-MS is here developed and validated for application to human and animal urine and hair samples. The method is based on the preliminary extraction of analytes by a magnetic multi-walled carbon nanotube poly(styrene-co-divinylbenzene) composite. This is followed by a < 9 min chromatographic separation of the target compounds in an Onyx Monolithic C18 column using a mixture of 0.01% (v/v) heptafluorobutyric acid in water and methanol at 500 µL min-1 flow rate. Detection limits within range from 0.055 to 0.093 µg mL-1, and precision values of the response and retention times of analytes were > 90%. Accuracy values comprised the range 79.5–109.5% when the analytes were extracted from deer urine samples using the selected MMWCNT-poly(STY-DVB) sorbent. This methodology was applied to real red deer urine and hair samples, the resulting concentrations within range from 0.05 to 0.5 µg mL-1for norepinephrine and from 1.0 to 44.5 µg mL-1 for its metabolite 3,4-dihydroxyphenyl glycol. Analyses of red deer hair resulted in high amounts of 3,4-dihydroxyphenyl. informacion[at]ebd.csic.es: Murtada et al (2019) LC-MS determination of catecholamines and related metabolites in red deer urine and hair extracted using magnetic multi-walled carbon nanotube poly(styrene-co-divinylbenzene) composite. J Chromatogr B https://doi.org/10.1016/j.jchromb.2019.121878


https://www.sciencedirect.com/science/article/abs/pii/S157002321931150X
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Content with tag catecholamines .

LC-MS determination of catecholamines and related metabolites in red deer urine and hair

A novel analytical methodology for the determination and extraction of catecholamines (dopamine, epinephrine and norepinephrine) and their metabolites DL-3,4-dihydroxyphenyl glycol and...

Unprecedented high catecholamine production causing hair pigmentation after urinary excretion in red deer

Hormones have not been found in concentrations of orders of magnitude higher than nanograms per milliliter. This study reports urine concentrations of a catecholamine (norepinephrine) ranging from...