Dogs are known to have originated from the gray wolf, with this domestication occurring during the Ice Age, at least 15,000 years ago. But where this happened, and if it occurred in one single location or in multiple places, is still unknown. Previous studies using the archaeological record and comparing the DNA of dogs and modern wolves have not found the answer. An international group of geneticists and archaeologists, led by the Francis Crick Institute and with the participation of Jennifer Leonard, a researcher at the Doñana Biological Station - CSIC, have found that the ancestry of dogs can be traced to at least two populations of ancient wolves. The work moves us a step closer to uncovering the mystery of where dogs underwent domestication, one of the biggest unanswered questions about human prehistory. 

In their study, published in Nature today (29 June), the researchers turned to ancient wolf genomes to further understanding of where the first dogs evolved from wolves. They analysed 72 ancient wolf genomes, spanning the last 100,000 years, from Europe, Siberia and North America. The remains came from previously excavated ancient wolves, with archaeologists from 38 institutions in 16 different countries contributing to the study. The remains included a full, perfectly preserved head from a Siberian wolf that lived 32,000 years ago. Nine different ancient DNA labs then collaborated on generating DNA sequence data from the wolves.

Two different sources

By analysing the genomes, the researchers found that both early and modern dogs are more genetically similar to ancient wolves in Asia than those in Europe, suggesting a domestication somewhere in the east. However, they also found evidence that two separate populations of wolves contributed DNA to dogs. Early dogs from north-eastern Europe, Siberia and the Americas appear to have a single, shared origin from the eastern source. But early dogs from the Middle East, Africa and southern Europe appear to have some ancestry from another source related to wolves in the Middle East, in addition to the eastern source.

One possible explanation for this dual ancestry is that wolves underwent domestication more than once, with the different populations then mixing together. Another possibility is that domestication happened only once, and that the dual ancestry is due to these early dogs then mixing with wild wolves. It is not currently possible to determine which of these two scenarios occurred. 

Anders Bergström, co-first author and post-doctoral researcher in the Ancient Genomics lab at the Crick, says: "Through this project we have greatly increased the number of sequenced ancient wolf genomes, allowing us to create a detailed picture of wolf ancestry over time, including around the time of dog origins."

"By trying to place the dog piece into this picture, we found that dogs derive ancestry from at least two separate wolf populations – an eastern source that contributed to all dogs and a separate more westerly source, that contributed to some dogs."

The role of Andalusian wolfs and dogs

The team are continuing the hunt for a close ancient wolf ancestor of dogs, which could reveal more precisely where domestication most likely took place. They are now focusing on genomes from other locations not included in this study, including more southerly regions. The Doñana Biological Station is continuing to collaborate in this research line. The next steps are both more extensive sampling of ancient Pleistocene wolves and Paleolithic and Chalcolithic dogs. "These samples are particularly important since the oldest gray wolf fossils are from southern Europe", explains Jennifer Leonard. "It is particularly difficult to get ancient DNA from ancient Andalucian bones because of the environment here which favors degradation of DNA." The ongoing collaboration between EBD and IAPH is particularly important to understand the place of Andalucian wolves and dogs in pre-history.

As the 72 ancient wolf genomes spanned around 30,000 generations, it was possible to look back and build a timeline of how wolf DNA has changed, tracing natural selection in action.

For example, they observed that over a period of around 10,000 years, one gene variant went from being very rare to being present in every wolf, and is still present in all wolves and dogs today. The variant affects a gene, IFT88, which is involved in the development of bones in the skull and jaw. It is possible that the spread of this variant could have been driven by a change in the types of prey available during the Ice Age, giving an advantage to wolves with a certain head shape, but the gene could also have other unknown functions in wolves.

Pontus Skoglund, senior author and group leader of the Ancient Genomics lab at the Crick, says: "This is the first time scientists have directly tracked natural selection in a large animal over a time-scale of 100,000 years, seeing evolution play out in real time rather than trying to reconstruct it from DNA today. We found several cases where mutations spread to the whole wolf species, which was possible because the species was highly connected over large distances. This connectivity is perhaps a reason why wolves managed to survive the Ice Age while many other large carnivores vanished." According to the researcher, similar whole-genome time series from the Ice Age, in humans or other animals, could provide new information about how evolution happens.

"This study highlights the dynamic evolutionary history of gray wolves across their range, and suggests human assisted geneflow could be appropriate to overcome human caused population fragmentation", concludes Jennifer Leonard. "Humans, wolves, and dogs have a long, intertwined history, so losing wolves as a functional component of our ecosystem would have cultural as well as ecological consequences". 

-----------

Reference:

Anders Bergström et al. Gray wolf genomic history reveals a dual ancestry of dogs. Nature. https://doi.org/10.1038/s41586-022-04824-9

Contacto:

Comunicación EBD

outreach@ebd.csic.es