We will introduce the different research activities carried out in the framework of eLTER Plus and SUMHAL projects concerning remote sensing upscaling in Doñana protected area. With the assistance of LAST-EBD and ICTS-RBD, both projects focus on the implementation and co-location of this 2 ESFRI Research Infrastructures in Doñana. A short intro on the long-term monitoring and research in Doñana will give way to present the methods developed to remote sensing monitoring of vegetation phenology and water and CO2 fluxes as well as products validation with in situ data.