A user-friendly tool, called SpotEgg, has been developed to analyse colour and pattering in digital images. The tool, operated through a Graphical User Interface, runs over Matlab 2012b or latter and was primarily aimed to analyse colouration in bird´s eggs. Nevertheless it can also measure reflectances and spottiness in any other type of objects as insects, feathers, frogs, etc. SpotEgg's main advantage is a custom designed spot detection algorithm. Additionally, it allows to reduce time-consuming routinary tasks when analysing digital images. The tool is available for downloading at the following webpage: https://www2.imse-cnm.csic.es/~linan/SPOTEGG/SpotEgg_Web_Site/main.html. informacion[at]ebd.csic.es: Gomez & Liñan-Cembrano (2016) SpotEgg: An image-processing tool for automatised analysis of colouration and spottiness. J Avian Biol doi: [10.1111/jav.01117]