Plant metabolic pathways and gene networks involved in the response to herbivory are well-established, but the impact of epigenetic factors as modulators of those responses is less understood. Mounting evidence indicates that epigenetic regulation can modulate plant responses that include priming and maternal effects on resistance. In this project, we aim to evaluate the role of DNA methylation as an epigenetic regulator in plant responses to herbivory by screening the effects of herbivory in DNA methylation, plant performance and defense in three non-model plant species (Thlaspi arvense, Fragaria vesca, Populus nigra). We investigate plants from diverse life histories and their phenotypic traits underpinned by insect and artificial herbivory induction to gain generality. Subsequently, we assess the correlation of phenotype/epigenotype variation using the Global DNA cytosine methylation by HPLC and Reduced Representation Bisulfite technique (epiGBS). Our study points towards multifaceted genetic-epigenetic interactions in determining herbivory-induced phenotypic plasticity and linking these epigenomic profiles to plant defensive traits could offer interesting opportunities for crop improvement.